Admin: Figure 1: Schematic and principle of scanned temporal focusing imaging system. (a) Schematic of the s-TeFo imaging. A large field-of-view is raster-scanned using an enlarged sculpted PSF and a one-pulse-per voxel excitation-acquisition scheme. Volumetr...

2016-11-01T15:00:48Z

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Figure 1: Schematic and principle of scanned temporal focusing imaging system. (a) Schematic of the s-TeFo imaging. A large field-of-view is raster-scanned using an enlarged sculpted PSF and a one-pulse-per voxel excitation-acquisition scheme. Volumetric image acquisition is achieved by translating the objective axially (along z-axis) via a high-speed long-range piezo. Scale bar is 100µm. (b) Measured axial confinement of the sculpted point-spread-function (PSF) of the laterally symmetric TeFo-spot using 0.5µm sized beads. Corresponding x and z profiles showing lateral and axial confinement of excitation, a.u., arbitrary units. Scale bar is 10µm. (c) Overview of the s-TeFo microscope. The main dichroic mirror and a mirror are mounted on a slide in order to switch between two-photon scanning and wide-field epi-fluorescent imaging mode. EOM, electro-optical modulator, BS, beam-splitter, PBS, polarizing beam-splitter.

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Admin: Figure 1: Schematic and principle of scanned temporal focusing imaging system. (a) Schematic of the s-TeFo imaging. A large field-of-view is raster-scanned using an enlarged sculpted PSF and a one-pulse-per voxel excitation-acquisition scheme. Volumetr...